B01：Development of ferromics probes for iron-specific imaging and proteomics
Tasuku HIRAYAMA
（Laboratory of Pharmeceutical and Medicinal Chemistry, Gifu Pharmaceutical University）
Lab website
In biological systems, iron ions and heme perform a variety of essential functions as protein-bound forms. In contrast, protein-free iron ions and heme (labile iron species) are also present in living cells, and changes in their concentration could be involved in the regulating system of cellular iron metabolism. It has been challenging to detect the labile iron species, and their behavior and functions are not fully understood. Our group has been actively developing fluorescent probes that can selectively detect intracellular labile iron (=Fe(II) ion). In this study, we will develop fluorescent probes for heme as well as high-performance probes for Fe(II). Additionally, we will develop novel chemical probes that are applicable to proteomics targeting Fe(II) ion and heme on the basis of our recently discovered chemical reaction. We hope that the probes that will be developed in this project will lead to a breakthrough in the iron-relevant life science.
Major publications
T. Hirayama, M. Inden, H. Tsuboi, M. Niwa, Y. Uchida, Y. Naka, I. Hozumi, H. Nagasawa,
“A Golgi-targeting fluorescent probe for labile Fe(II) to reveal an abnormal cellular iron distribution induced by dysfunction of VPS35”
Chem. Sci., 2019, 10, 1514–1521
doi: 10.1039/C8SC04386H
M. Niwa, T. Hirayama, I. Oomoto, D. O. Wang, H. Nagasawa
“Fe(II) ion release during endocytotic uptake of iron visualized by a membrane-anchoring Fe(II) fluorescent probe”
ACS Chem. Biol., 2018, 13, 1853–1861
doi: 10.1021/acschembio.7b00939
T. Hirayama, H. Tsuboi, M. Niwa, A. Miki, S. Kadota, Y. Ikeshita, K. Okuda and H. Nagasawa
“Fe(II) ion release during endocytotic uptake of iron visualized by a membrane-anchoring Fe(II) fluorescent probe”
Chem. Sci., 2017, 8, 4858–4866
doi: 10.1039/C6SC05457A
T. Hirayama, K. Okuda and H. Nagasawa
“A highly selective turn-on fluorescent probe for iron(II) to visualize labile iron in living cells”
Chem. Sci., 2013, 4, 1250–1256
doi: 10.1039/c2sc21649c