A01-2:Construction and Physiological Function of Biological Sensory Systems Regulated by in vivo Biometal Dynamics
Shigetoshi Aono
(Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences)

Lab website

Biological systems for sensing external signals and signal transduction often require metal ions as essential elements for their functional expression. It plays an important role for the biogenesis of these sensory and signal transduction systems that various accessory and chaperone proteins regulate the assembly and transport of metal ions/metal complexes in vivo. In this research, we will elucidate the molecular mechanisms the biogenesis of sensor proteins that contain transition metal ions in their sensor modules to understand a basic principle of the construction of biological sensory systems regulated by in vivobiometal dynamics.The structural dynamics and the physiological function of the biological gas sensing systems will be elucidated by spectroscopy, X-ray crystallography, and biochemical/molecular biological analyses.

Major publication
C. Kitatsuji, M. Ogura, T. Uchida, K. Ishimori, and *S. Aono
“Molecular mechanism for heme-mediated inhibition of 5-aminolevulinic acid synthase 1”
Bull. Chem. Soc. Jpn., 2014, 997-1004
doi: 10.1246/bcsj.20140111

H. Sawai, H. Sugimoto, Y. Shiro, H. Ishikawa, Y. Mizutani, and *S. Aono
“Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa
Chem. Commun., 2012, 48, 6523-6525
doi: 10.1039/c2cc32549g

*S. Aono
“Novel bacterial gas sensor proteins with transition-metal-containing prosthetic groups as active sites”
Antioxd. Redox Signal., 2012, 16, 678-686
doi: 10.1089/ars.2011.4248

H. Yoshimura, S. Yoshioka, K. Kobayashi, T. Ohta, T. Uchida, M. Kubo, T. Kitagawa, and *S. Aono
“Specific hydrogen bonding networks responsible for selective O2 sensing for the oxygen sensor protein HemAT from Bacillus subtilis
Biochemistry, 2006, 45, 8301-8307
doi: 10.1021/bi060315c

S. Inagaki, T. Ohta, T. Kitagawa, and *S. Aono
“Spectroscopic and redox properties of a CooA homologue from Carboxydothermus hydrogenoformans
J. Biol. Chem., 2005, 280, 3269-3274
doi: 10.1074/jbc.M409884200