B01-4：Biochemical Imaging with quantum nanobeam for Dynamics analysis of Biometals
Shino Takeda
（National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology）
Lab website
It has been pointed out that the conventional elemental analysis method by destroying or dissolving tissues may not reflect the in vivo condition. In this project, as a research tactic to understand biometal dynamics in situ, we work on establishment of bio-chemical imaging using quantum nanobeam and construction of intracellular biometal dynamics characteristics. In particular, the purpose is to elucidate the formation mechanism of localized and concentrated biometals by combination of hyperspectral image to grasp precise tissue or cell structure and nanobeam based elemental analysis, such as particle induced X-ray emission (PIXE), synchrotron radiation X-ray fluorescence (SR-XRF) and X-ray absorption fine structure (XAFS) to obtain biometal distribution and chemical status.
Major publications
S. Homma-Takeda, A. Uehara, T. Yoshida, C. Numako, O. Sekizawa, K. Nitta, and N. Sato
“Two-dimensional μXAFS for uranium accumulated in kidney of rats exposed to uranyl acetate”
Radiation Physics and Chemistry, 2019, in press
doi: 10.1016/j.radphyschem.2019.02.006
K. Kitahara, C. Numako, Y. Tearada, K. Nitta, Y. Shimada, and S. Homma-Takeda
“Uranium XAFS analysis of kidney from rats exposed to uranium”
Journal of Synchrotron Radiation 2017, 24, 456-462
doi: 10.1107/S1600577517001850
S. Homma-Takeda, K. Kitahara, K. Suzuki, B. J. Blyth, N. Suya, T. Konishi, Y. Terada, and Y. Shimada
“Cellular localization of uranium in the renal proximal tubules during acute renal uranium toxicity”
Journal of Applied Toxicology 2015, 35, 1594-1600
doi: 10.1002/jat.3126
S. Homma-Takeda, T. Kokubo, Y. Terada, K. Suzuki, S. Ueno, T. Hayao, T. Inoue, K. Kitahara, B. J. Blyth, M. Nishimura, and Y. Shimada
“Uranium dynamics and developmental sensitivity in rat kidney”
Journal of Applied Toxicology 2013, 33, 685-694
doi: 10.1002/jat.2870
S. Homma-Takeda, Y. Nishimura, H. Iso, T. Ishikawa, H. Imaseki, and M. Yukawa
“A new approach for standard preparation in microbeam analysis: Development and validation”
Journal of Radioanalytical and Nuclear Chemistry 2009, 279, 627-631
doi: 10.1007/s10967-008-7312-6