A03：Elucidation of the mechanisms underlying iron-dependent-ferroptosis
Hiroaki FUJITA
Graduate School of Medicine, University of Kyoto
Lab website
Iron behaves as a double-edged sword, essential for living but also potentially toxic through the generation of oxidative stress. Recently, it has been reported that Ferroptosis is a novel type of programmed cell death dependent on iron and characterized by the accumulation of lipid peroxides. Previously the mechanisms that suppress lipid peroxidation have been extensively studied. However, the mechanisms how iron is involved in ferroptosis remain elusive. Here, to uncover the mechanisms underlying iron-dependent-ferroptosis, we developed some KO cell lines of iron regulator by using CRISPR/Cas9 and analyze the role of iron in ferroptosis.
Major publications
H. Fujita, A. Tokunaga, S. Shimizu, AL. Whiting, F. Aguilar-Alonso, K. Takagi, E. Walinda, Y. Sasaki, T. Shimokawa, T. Mizushima, I. Ohki, M. Ariyoshi, H. Tochio, F. Bernal, M. Shirakawa, K. Iwai
“Cooperative domain formation by homologous motifs in HOIL-1L and SHARPIN plays crucial roles in LUBAC stabilization”
Cell reports 2018, 23, 1192-1204
doi: 10.1016/j.celrep.2018.03.112
H. Fujita, S. Rahighi, M. Akita, R. Kato, Y. Sasaki, S. Wakatsuki, K. Iwai
“Mechanism underlying IKK activation mediated by the linear ubiquitin chain assembly complex (LUBAC)”
Mol. Cell. Biol. 2014, 34, 1322-1335
doi: 10.1128/MCB.01538-13
K. Iwai, H. Fujita, Y. Sasaki
“Linear ubiquitin chains: NF-κB signalling, cell death, and beyond.”
Nature Rev. Mol. Cell Biol. 2014, 15, 503-508
doi: 10.1038/nrm3836