B01：Development of Single Cell Handling and Sampling Devices for Biometal Science
Tomonari UMEMURA
（School of Life Sciences, Tokyo University of Pharmacy and Life Sciences）
Lab website
What kinds of and how many elements (trace metals) are contained in a single cell? Such a seemingly simple thing has not been revealed yet. The combination of different metals may exhibit an additive, synergistic, or antagonistic effect on cellular functions. Also, recent single-cell analysis reveals cellular heterogeneity and dynamics of individual cells. Therefore, there is a great need for comprehensive genome-wide multielement analysis of single cells. In the present study, we will construct two types of microfabricated devices for single cell multielement analysis by inductively coupled plasma mass spectrometry; 1) a multi-well plate for trapping and rinsing of single cells and 2) a microfluidic device for aligning and delivering single cells.
Major publications
H. Tani,, A. Numajiri, M. Aoki, T. Umemura, T. Nakazato br> “Short-lived Long Noncoding RNAs as Surrogate Indicators for Chemical Stress in HepG2 Cells and Their Degradation by Nuclear RNases”
Sci. Rep., 2019, 9, 20299
doi: 10.1038/s41598-019-56869-y
E. Fujimori, S. Nagata, H. Kumata, T. Umemura
“Investigation of Adverse Effect of Coexisting Aminopolycarboxylates on the Determination of Rare Earth Elements by ICP-MS After Solid Phase Extraction Using an Iminodiacetate-Based Chelating-Resin”
Chemosphere 2019, 214, 288-294
doi: 10.1016/j.chemosphere.2018.09.073
A. Hirose, T. Kasai, M. Aoki, T. Umemura, K. Watanabe, A. Kouzuma
“Electrochemically Active Bacteria Sense Electrode Potentials for Regulating Catabolic Pathways”
Nat. Commun., 2018, 9, 1083
doi: 10.1038/s41467-018-03416-4